Euglena viridis S 1

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: wastewater treatment plants of Almaty
• Habitat: wastewater
• Isolator: Zayadan B.K., Akmukhanova N.R. at the Biotechnology Laboratory of Al-Farabi Kazakh National University.
• Year of accession to the collection: 2001.
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type (WT)
• Strain condition: axenic

Morphology and Structure

Cells are spindle-shaped, the cells exhibit high metaboly, the anterior end is obliquely rounded, the posterior end is narrowed with a colorless point. The cell is enclosed in a cytoplasmic membrane, to which an elastic, spirally striated periplast is adjacent from the outside. The cell contains one large stellate chromatophore located in the center of the protoplast and containing a pyrenoid with a paramylon sheath. In the posterior half of the cell, one large oval nucleus is localized. The organism swims with the help of a single flagellum located at the anterior end, the length of which is approximately equal to the length of the cell.

Cultivation

• Nutrient Medium: BG-11 medium (liquid and agarized)
• Temperature: 25–28 °C
• Light Intensity: 40–80 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 6.5–7.0

Physiological Characteristics

neutrophile, mesophile, autotroph, photophilic.

Long-term Preservation Conditions

maintained on liquid and 1.5–2.0% agarized BG-11 medium. The preservation temperature is 10–12 °C, light intensity is 10–20 µmol photons/m²/s, with a 12:12 h (light/dark) photoperiod. Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

deposited in 2003 into the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration Number: A-RKM 0133

Field of Application

in fundamental research and biotechnology.