Chlorella vulgaris SP BB-2

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: contaminated Lake Bilikol, southern Republic of Kazakhstan (42°59′18′′ N, 70°41′14′′ E)
• Habitat: contaminated water body (lake)
• Isolator(s): Torekhanova M.M., Zayadan B.K., Akmukhanova N.R., Sadvakasova A.K., Bolatkhan K., Zayadan B.K. in the biotechnology laboratory of Al-Farabi KazNU
• Year of accession to the collection: 2015
• Last identification: Zayadan B.K., Torekhanova M.M., Sadvakasova A.K., 2022
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type
• Strain condition: axenic

Morphology and Structure

unicellular green microalga Chlorella vulgaris SP BB-2; cells spherical, small, green; characterized by a high degree of wastewater treatment, productivity, and significant content of valuable compounds in biomass

Cultivation

• Culture medium: Tamiya mineral medium (liquid and agarized)
• Temperature: 24–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH of the medium: 6.5

Physiological Characteristics

mesophilic, autotrophic, and neutrophilic; capable of active growth in wastewater contaminated with mineral and organic compounds; exhibits a high capacity for nutrient removal and reduction of organic pollutants from wastewater.

Long-term Preservation Conditions

Stored on liquid and 1.5–2.0 % agarized Tamiya mineral medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

certificate of deposit of a microorganism No. 09/12-310; deposited in 2016 in the Republican Collection of Microorganisms of the Republic of Kazakhstan. Collection number assigned by the collection — RKM 0713

Field of Application

biotechnology, environmental protection; production of biomass as biologically active additives and treatment of wastewater from organic and mineral pollutants; obtainment of a microalgal strain capable of active growth in wastewater contaminated with mineral and organic impurities

Bibliography

1. Заядан Б.К., Акмуханова Н.Р., Тореханова М.М., Садвакасова А.К., Бауенова М.О. Биологическая очистка рыбохозяйственных сточных вод на основе микроводорослей Chlorella vulgaris sp. BB-2 // Аспекты и инновации биотехнологии окружающей среды и биоэнергетики: сборник материалов Международной научно-практической конференции, Алматы, 12–13 февраля 2021 г. – Алматы: Қазақ университеті, 2021. – С. 159–163.

Patents and Other Intellectual Property

Invention patent No. 35781. Application No. 2021/0211.1 dated March 30, 2021. Publication date: September 30, 2022, Bulletin No. 39. Title: "Strain of the microalga Chlorella vulgaris sp BB-2, promising for biomass production and wastewater treatment to remove organic-mineral contaminants." Patent holder: Non-profit Joint-Stock Company "Al-Farabi Kazakh National University." Authors: Torekhanova Makpal Merkibekovna; Sadvakasova Asemgul Kalyikumarovna; Akmukhanova Nurziya Rakhmedievna; Bolatkhan Kenzhegul; Zayadan Bolatkhan Kazykhanuly.