Dunaliella salina B-10

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: East Kazakhstan Region, Lake Alakol
• Habitat: brackish waters of Lake Alakol
• Isolator(s): B.K. Zayadan & et al.
• Year of accession to the collection: 2021
• Method of strain acquisition: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking.
• Genetic features: Wild type (WT)
• Culture state: axenic

Morphology and Structure

The cells of the strain exhibit diverse shapes, including oval, ellipsoidal, ovoid, pyriform, occasionally spherical, cylindrical, or fusiform; they are radially or bilaterally symmetrical, rarely dorsoventral or slightly asymmetrical, possessing two equal smooth flagella at the anterior convex end, with average dimensions of 16 × 22 μm. The chloroplast color is most commonly green, but occasionally varies from yellow or brownish to red.

Cultivation

• Nutrient medium: BG-11 medium (liquid and agar-solidified)
• Temperature: 25–28 °C
• Light intensity: 50–100 µmol photons/m²/s
• Light regime: 16:8 h (light/dark)
• pH: 7.5

Physiological Characteristics

mesophile, photoautotroph.

Long-term Preservation Conditions

maintained on liquid and 1.5–2.0% agarized mineral BG-11 medium. Storage temperature: 12–15 °C; light intensity: 5–10 μmol photons/m²/s; photoperiod: 12:12 h (light/dark). Culture subculturing is performed every 3 months.

Deposition Information

certificate of deposition of microorganism No. 09/12-398 in 2021 into the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration Number: RKM 0962

Field of Application

in fundamental research and biotechnology.