Spirulina labyrinthiformis L-2

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: wastewater treatment plants of Almaty.
• Habitat: wastewater
• Isolator: Zayadan B.K., Akmukhanova N.R. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2001
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Strain condition: axenic

Morphology and Structure

The trichomes are pale blue-green and form a geometrically regular spiral. The coils of this spiral have a diameter of 2.0–2.7 µm and are tightly attached to each other. During biomass cultivation, the trichomes form characteristic dirty black-green clusters.

Cultivation

• Nutrient Medium: Zarrouk’s medium (liquid and agarized)
• Temperature: 27–30 °C
• Light Intensity: 50–150 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 8.0–10.0

Physiological Characteristics

mesophile, autotroph.

Long-term Preservation Conditions

maintained on liquid and 1.5–2.0% agarized Zarrouk’s medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Deposition Information

deposited in 2003 into the Republican Collection of Microorganisms of the Republic of Kazakhstan. Registration Number: A-RKM 0116

Field of Application

in fundamental research and biotechnology.