Synechocystis sp. K-1

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: Almaty Region, Chonji district.
• Habitat: thermal springs.
• Isolator(s): Zayadan B.K., Akmukhanova N.R. in the Biotechnology Laboratory of Al-Farabi Kazakh National University.
• Year of accession to the collection: 2010.
• Method of obtainment: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Culture state: axenic

Morphology and Structure

prokaryotic, blue-green in color.

Cultivation

• Nutrient Medium: Gromov No. 6 medium (liquid and agarized)
• Temperature: 26–28 °C
• Light Intensity: 50–150 µmol photons/m²/s
• Photoperiod: 16:8 h (light/dark)
• pH: 6.5–7.0

Physiological Characteristics

neutrophile, autotroph, photophilic, nitrogen-fixing. Under optimal cultivation conditions, 2.0–2.5 g/L dry biomass is obtained on days 7–8; proteins 25–30%, carbohydrates 48–56%.

Long-term Preservation Conditions

maintained on liquid and 1.5–2.0% agarized Gromov No. 6 medium. Storage temperature 10–12 °C, light intensity 10–20 µmol photons/m²/s, photoperiod 12:12 h (light/dark). Subculturing is carried out as needed, but at least once every 2–4 months.

Field of Application

in fundamental research and biotechnology.