Phormidium autumnale I-5

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan
• Place of isolation: Ilek River, Aktobe city, Aktobe region
• Habitat: freshwater river ecosystem
• Isolator(s): Zayadan B.K., Akmukhanova N.R., Sadvakasova A.K., Bauenova M.O. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2016.
• Method of obtainment: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Strain condition: axenic.

Morphology and Structure

Trichomes are curved, intertwined with each other, constricted in the area of the transverse septa, and are light blue-green, 0.6–0.8 µm wide. The sheaths are colourless and stick together. The cells are cylindrical; their length is 2–8 times greater than their width; granulations in the area of the transverse septa are absent. The threads have a uniform thickness along their entire length, with only the very tip slightly tapered and slightly curved. The terminal cell is blunt-conical. Division occurs in a single plane, resulting in the cells being arranged in a linear order. The colour is blue-green. The cells do not settle to the bottom; the culture grows, forming a film on the walls of the culture vessel.

Cultivation

• Culture medium: Zarrouk medium (liquid and agarized)
• Temperature: 22–30 °C (optimum 25 °C)
• Light intensity: 60–70 µmol photons·m⁻²·s⁻¹
• Light regime: 12:12 h (light/dark)
• pH of the medium: 6.5–7.0

Physiological Characteristics

Photoautotroph; mesophile.

Long-term Preservation Conditions

stored on solid Prat medium at a temperature of 12–14 °C and low light 10–20 µmol photons·m⁻²·s⁻¹. Maximum storage period — 2 months.

Deposition Information

Certificate of deposition No. 09/12-308 dated 2016 in the RSE on the REM "Republican Collection of Microorganisms" of the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan. Registration Number: RKM 711.

Field of Application

In fundamental research and biotechnology.

Bibliography

1. Formation of structured biocenoses of higher aquatic plants and phototrophic microorganisms for use in wastewater treatment. Akmukhanova N.R., Zayadan B.K., Bauenova M.O., Sadvakasova A.K., Bolatkhan K., Seiilbek S. Eurasian Journal of Ecology. No. 3 (52). 2017.