Nostoc calcicola TSZ 2203

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan.
• Place of isolation: hot springs of Chundzha, Almaty Region
• Habitat: Thermal aquatic ecosystem.
• Isolator(s): Sandybayeva S.K., Zayadan B.K., Kakimova A.B., Bolatkhan K., Toktybay A.K., Seiilbek S.N. at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2023.
• Method of obtainment: isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking
• Genetic features: Wild type (WT)
• Culture condition: axenic.

Morphology and Structure

Filamentous cyanobacterium with intercalary and terminal heterocytes. Heterocytes are ovoid, spherical or ellipsoidal, 1.54–3.37 µm wide and 3.21–6.19 µm long. Akinetes are formed centrifugally in rows between heterocytes.

Cultivation

• Nutrient medium: BG-11₀ medium without combined nitrogen
• Temperature: 28 ± 2 °C
• Light intensity: 53 µmol photons·m⁻²·s⁻¹
• Photoperiod: 12:12 h (light/dark)
• pH: 7.5

Physiological Characteristics

photoautotroph; heterocytous nitrogen-fixing cyanobacterium; aerobic phototroph

Long-term Preservation Conditions

Maintained in liquid BG-11₀ medium with transfer every 2–3 months. Dried biomass is stored at 4 °C in darkness under dry conditions.

Deposition Information

Certificate of deposit No. 09/12-250 dated October 19, 2023. Registration Number: RKM 1061

Genetic Information

Molecular identification was based on 16S rRNA gene sequencing using primers 27F/1492R. GenBank accession: № OQ627023.1

Field of Application

Fundamental studies of heterocyte differentiation and nitrogen fixation; screening as a potential source of pigments, protein, fatty acids and other metabolites.

Bibliography

1. Sandybayeva, S.K.; Kossalbayev, B.D.; Zayadan, B.K.; Kopecký, J.; Kakimova, A.B.; Bolatkhan, K.; Allakhverdiev, S.I. Isolation, Identification and Pigment Analysis of Novel Cyanobacterial Strains from Thermal Springs. Plants 2024, 13, 2951. https://doi.org/10.3390/plants13212951

Patents and Other Intellectual Property

None.