Nostoc sp.

History of Receipt and Isolation

• Country of origin: Republic of Kazakhstan.
• Place of isolation: Aktau, industrial zone of the Oil Refinery, production units, sampling point M-4 (at the outlet of the production units)
• Habitat: Oil-contaminated wastewater
• Isolator(s): B.K. Zayadan, A.A. Dauletova, K.Bolatkhan at the Biotechnology Laboratory of Al-Farabi Kazakh National University
• Year of accession to the collection: 2024.
• Method of obtainment: Isolated from an enrichment culture obtained from a natural sample by sequential microbiological streaking onto solid nitrogen-free nutrient medium
• Genetic features: Wild type (WT)
• Culture condition: Axenic

Morphology and Structure

Filamentous colonial prokaryotic organism (cyanobacterium). Cells are spherical or barrel-shaped, lacking flagella, and connected into bead-like unbranched filaments (trichomes) surrounded by a mucilaginous sheath. Forms slimy colonies. Under nitrogen deficiency, it differentiates large spherical heterocysts (6–10 µm). The size of vegetative cells ranges from 3 to 7 µm, classifying the strain within the group of medium-sized microorganisms (2–20 µm).

Cultivation

• culture medium: BG-11 medium; modified nitrogen-free BG-11 medium (liquid and agarized)
• temperature: 25–28 °C
• light intensity: 40–60 µmol photons/m²/s
• photoperiod: 16 h light / 8 h darkness
• pH of the medium: 7.5–8.0

Physiological Characteristics

Halotolerant.

Long-term Preservation Conditions

maintained as a subculture on liquid and 1.5–2.0% agarized nitrogen-free mineral BG11₀ medium. Storage temperature is +12…+15 °C, light intensity is minimal (5–10 µmol photons/m²/s), and the photoperiod is 12:12 h (light/dark). Subculturing of the collection culture is performed every 4–6 months.

Genetic Information

GenBank Accession №PZ747267

Field of Application

Utilized in fundamental research and biotechnology.